P-340 Endometrial microbiome - is every dysbiosis an inflammation?

Abstract Study question The endometrial microbiome, in addition to other parameters of inflammation (CD138 presence, NK bright fraction presence), may be a complementary marker ongoing local inflammation. Summary answer Presented results the microbiome comparison with CD 138 indicate endometrium, which is caused by dysbiosis. What known already study and assessment its impact on female reproductive system embryo implantation, pregnancy maintenance success vitro fertilization (IVF) procedure an increasingly popular research topic. protective role Lactobacillus (other than L.iners) known, probably low number related impossibility implantation. Dysfunctional mycobiome cause chronic including endometriosis recurrent implantation failures. Importantly, dysbiotic profile microflora often does not any clinical symptoms. design, size, duration included 182 women child-bearing age, who were patients Gyncentrum Clinic (Poland), qualified for IVF procedure. In this group patients, 201 samples combined tested, obtaining 19 negative due quality swabs, small amount microbiota impossible efficiently isolate. study, analysed from January December 2022 collection. Participants/materials, setting, methods To analyze assumed parameters, treated at underwent routine hysteroscopy procedure, during swabs (determination molecular using Next Generation Sequencing NGS method) tissue fragments CD138 immunohistochemically fractions flow cytometry) collected. Nucleic acids isolated preparation libraries, based Illumina-16S Metagenomic Library Preparation. Main chance our allowed us observe some trends. Due microbiological endometrium profile, divided into 3 groups: 1) normal (domination L.iners and/or Bifidobacterium; n = 135), 2) moderate dysbiosis (presence Bifidobacterium L. iners-max. 50% all sample- also potentially harmful species e.g. Enterobacteriaceae group, Streptococcus Veilonella; 28); 3) (with/ without Bifidobacterium, strong dominance listed above or dominance; 19). We observed that increased index (⩾1/10 high power fields HPF) more noted - 0/10 HPF 7.21%, 15.49%. didn`t notice trends comparing (CD56 ++/CD16-) tested specimens, relationship microbiome. Observed trend indicates certain presence abnormal expressed CD138, but it requires confirmation larger obtain information about pregnancy. Limitations, reasons caution conducted require broader analysis patients. It necessary full analyzed Identification taxa method same as identification microorganisms living studied microenvironment. Wider implications findings should starting point further analyses, important context infertility, focusing relationships between effectiveness. seems interesting extend elements viruses, fungi, protozoa. Trial registration 161/KBL/OIL/2021